CLSI VET03 A Ed. 1 (2006)

CLSI VET03 A Ed. 1 (2006) Methods For Antimicrobial Disk Susceptibility Testing Of Bacteria Isolated From Aquatic Animals Provides the most up-to-date techniques for disk diffusion susceptibility testing of aquatic species isolates, and criteria for quality control testing.

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Table of Contents

Abstract
CommitteeMembership
Foreword
1Scope
2Introduction
3Definitions
4IndicationsforPerformingSusceptibilityTesting
5SelectionofAntimicrobialAgentsforRoutineTesting
andReporting
5.1RoutineReports
5.2AntimicrobialClasses
5.3SuggestedGuidelinesforUseandSelectiveTesting
andReporting
6DiskDiffusionAssayProtocol
6.1MediaPreparation
6.2Inoculum
6.3DiffusionDisks
6.4Incubation
6.5ReadingPlates
6.6RejectionCriteria
7FastidiousandProblemOrganisms
7.1VibrionaceaeandPhotobacteriaceae(ObligateHalophilic
Strains)(Group2)
7.2GlidingBacteria(Group3)
7.3Streptococci(Group4)
7.4OtherFastidiousOrganisms(Group5)
8QualityControlProcedures
8.1Purpose
8.2ReferenceStrainsforQualityControl
8.3AcceptedQualityControlRanges
8.4ExpansionofControlStrainSet
8.5StoringandTestingQualityControlStrains
8.6ControlofMediaand
8.7FrequencyofQualityControlTesting
8.8CorrectiveAction
9QualityAssuranceMeasures
References
AppendixA-AntimicrobialAgentsUsedinGlobalAquaculture
andStatusofQualityControlforDiskDiffusion
SusceptibilityTesting
AppendixB-FuzzyZonesandHaloFormation
AppendixC-DiskDiffusionDailyQualityControlTesting
Protocol
AppendixD-DiskDiffusionWeeklyQualityControlTesting
Protocol
SummaryofDelegate/ConsensusCommentsandWorkingGroup
Responses
TheQualitySystemApproach
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Abstract

Provides the most up-to-date techniques for disk diffusion susceptibility testing of aquatic species isolates, and criteria for quality control testing.

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