Table of Contents
Abstract
CommitteeMembership
Foreword
1Scope
2Introduction
3Definitions
4AntimicrobialAgents
4.1Source
4.2WeighingAntimicrobialPowders
4.3PreparingStockSolutions
4.4NumberofConcentrationsTested
5SelectionofAntimicrobialAgentsforRoutineTesting
andReporting
5.1RoutineReports
5.2AntimicrobialClasses
5.3SuggestedGuidelinesforUseandSelectiveTesting
andReporting
6IndicationsforPerformingSusceptibilityTesting
7InoculumPreparationforDilutionTests
7.1TurbidityStandardforInoculumPreparation
7.2DirectColonySuspensionMethod
8BrothDilutionProcedures(MacrodilutionandMicrodilution)
8.1Cation-AdjustedMueller-HintonBroth(CAMHB)Medium
8.2PreparingandStoringDilutedAntimicrobialAgents
8.3BrothDilutionTesting
9FastidiousandProblemOrganisms
9.1VibrionaceaeandPhotobacteriaceae(ObligateHalophilic
Strains)(Group2)
9.2GlidingBacteria(Group3)
9.3Streptococci(Group4)
9.4OtherFastidiousOrganisms(Group5)
10ReportingofMICResults
11QualityControlProcedures
11.1Purpose
11.2QualityControlResponsibilities
11.3ReferenceStrainsforQualityControl
11.4StoringandTestingQualityControlStrains
11.5ControlofMediaandMaterials
11.6BrothDilutionQualityControlRanges
11.7FrequencyofQualityControlTesting
11.8CorrectiveAction
11.9OtherControlProcedures
12LimitationsofBrothDilutionTestMethods
12.1ApplicationtoVariousOrganismGroups
References
AppendixA-AntimicrobialAgentsUsedinGlobalAquaculture
andStatusofQualityControlforBrothDilution
SusceptibilityTesting
AppendixB-AerobicDilutionDailyQualityControlTesting
Protocol
AppendixC-AerobicDilutionWeeklyQualityControlTesting
Protocol
SummaryofCommentsandSubcommitteeResponses
TheQualitySystemApproach
RelatedCLSI/NCCLSPublications Abstract
Provides the most up-to-date techniques for the determination of minimal inhibitory concentrations (MICs) of aquatic bacteria by broth micro- and macrodilution, and criteria for quality control testing.
